lysosomal associated membrane protein 2 Search Results


baf atpase function  (MedChemExpress)
94
MedChemExpress baf atpase function
(A) Schematic representation of three biochemically distinct <t>BAF</t> complexes: nBAF (neuronal cBAF), GBAF, and PBAF. Complex-defining unique subunits are represented in colors. Subunits shared by all three complexes are not indicated. Degraders and small-molecule inhibitors to target each complex are noted below. Schematic was created using BioRender. (B) Neurons were treated with DMSO (control) or the indicated concentrations of ACBI1 or cis -ACBI1 (inactive isomer of ACBI1) for 3 h. Whole-cell lysates were electrophoresed, western blotted, and probed for the indicated BAF subunits. (C–E) Neuronal lysates were used to immunoprecipitate SMARCA4 (C), ARID1A (D), and ARID1B (E). Five percent of the cell lysate was used as input. Histone 3 and IgG are depicted as loading controls. (F and G) Transcriptional assays in which Arc pre-mRNA normalized by GAPDH pre-mRNA levels is illustrated. (F) Neurons were treated with ACBI1 or cis -ACBI1 for 3 h followed by bicuculline and 4AP treatment for 15 min (Bic + 4AP). (G) Neurons were treated with BAF <t>ATPase</t> domain inhibitors BRM014 and FHT for 30 min, followed by Bic + 4AP treatment to induce neuronal activity. Gray dots represent biological replicates, error bars show SE of the mean. * p < 0.05 and ** p < 0.01. One-way ANOVA was followed by Tukey’s post hoc test. Approximate position of the nearest molecular weight marker is depicted against each band.
Baf Atpase Function, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/ATP6AP2%2C+Human/pmc11625021-355-2-8
Average 94 stars, based on 1 article reviews
baf atpase function - by Bioz Stars, 2026-09
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lamp2  (Boster Bio)
94
Boster Bio lamp2
(A) Schematic representation of three biochemically distinct <t>BAF</t> complexes: nBAF (neuronal cBAF), GBAF, and PBAF. Complex-defining unique subunits are represented in colors. Subunits shared by all three complexes are not indicated. Degraders and small-molecule inhibitors to target each complex are noted below. Schematic was created using BioRender. (B) Neurons were treated with DMSO (control) or the indicated concentrations of ACBI1 or cis -ACBI1 (inactive isomer of ACBI1) for 3 h. Whole-cell lysates were electrophoresed, western blotted, and probed for the indicated BAF subunits. (C–E) Neuronal lysates were used to immunoprecipitate SMARCA4 (C), ARID1A (D), and ARID1B (E). Five percent of the cell lysate was used as input. Histone 3 and IgG are depicted as loading controls. (F and G) Transcriptional assays in which Arc pre-mRNA normalized by GAPDH pre-mRNA levels is illustrated. (F) Neurons were treated with ACBI1 or cis -ACBI1 for 3 h followed by bicuculline and 4AP treatment for 15 min (Bic + 4AP). (G) Neurons were treated with BAF <t>ATPase</t> domain inhibitors BRM014 and FHT for 30 min, followed by Bic + 4AP treatment to induce neuronal activity. Gray dots represent biological replicates, error bars show SE of the mean. * p < 0.05 and ** p < 0.01. One-way ANOVA was followed by Tukey’s post hoc test. Approximate position of the nearest molecular weight marker is depicted against each band.
Lamp2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/Anti-LAMP2a+Rabbit+Monoclonal+Antibody/pmc12907057-303-47-48
Average 94 stars, based on 1 article reviews
lamp2 - by Bioz Stars, 2026-09
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lamp2  (ProSci Incorporated)
90
ProSci Incorporated lamp2
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Lamp2, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/LAMP2+Antibody/pmc05520723-138-8-12
Average 90 stars, based on 1 article reviews
lamp2 - by Bioz Stars, 2026-09
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rat monoclonal anti lamp 2  (StressMarq)
92
StressMarq rat monoclonal anti lamp 2
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Rat Monoclonal Anti Lamp 2, supplied by StressMarq, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/Anti-LAMP2+Antibody/pmc11289521-262-49-54
Average 92 stars, based on 1 article reviews
rat monoclonal anti lamp 2 - by Bioz Stars, 2026-09
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antibody specific to human lamp1  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare antibody specific to human lamp1
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Antibody Specific To Human Lamp1, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/lysosome+associated+membrane+protein+2++lamp2+++abl+93++antibody/pm20522958-193-24-7
Average 90 stars, based on 1 article reviews
antibody specific to human lamp1 - by Bioz Stars, 2026-09
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anti-lamp2 rabbit polyclonal antibody  (Bioworld Antibodies)
90
Bioworld Antibodies anti-lamp2 rabbit polyclonal antibody
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Anti Lamp2 Rabbit Polyclonal Antibody, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/lysosomal+associated+membrane+protein+2++lamp+2/ppr0282580-59-12-18
Average 90 stars, based on 1 article reviews
anti-lamp2 rabbit polyclonal antibody - by Bioz Stars, 2026-09
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primary antibodies against human cd107b (lysosome-associated membrane protein-2, lamp-2) #10-672-c100  (HiSS Diagnostics)
90
HiSS Diagnostics primary antibodies against human cd107b (lysosome-associated membrane protein-2, lamp-2) #10-672-c100
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Primary Antibodies Against Human Cd107b (Lysosome Associated Membrane Protein 2, Lamp 2) #10 672 C100, supplied by HiSS Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
primary antibodies against human cd107b (lysosome-associated membrane protein-2, lamp-2) #10-672-c100 - by Bioz Stars, 2026-09
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elisa kit for lysosomal associated membrane protein 2 (lamp2; cat. # seb464hu)  (Cloud-Clone corp)
90
Cloud-Clone corp elisa kit for lysosomal associated membrane protein 2 (lamp2; cat. # seb464hu)
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Elisa Kit For Lysosomal Associated Membrane Protein 2 (Lamp2; Cat. # Seb464hu), supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit for lysosomal associated membrane protein 2 (lamp2; cat. # seb464hu) - by Bioz Stars, 2026-09
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anti-lamp-2(lysosomal-associated membrane protein 2) pe  (Bioquote Inc)
90
Bioquote Inc anti-lamp-2(lysosomal-associated membrane protein 2) pe
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Anti Lamp 2(lysosomal Associated Membrane Protein 2) Pe, supplied by Bioquote Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-lamp-2(lysosomal-associated membrane protein 2) pe - by Bioz Stars, 2026-09
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rat anti-lysosome–associated membrane protein-2 antibody  (Becton Dickinson)
90
Becton Dickinson rat anti-lysosome–associated membrane protein-2 antibody
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Rat Anti Lysosome–Associated Membrane Protein 2 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-lysosome–associated membrane protein-2 antibody - by Bioz Stars, 2026-09
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lamp2a  (MedChemExpress)
93
MedChemExpress lamp2a
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Lamp2a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/LAMP2+Antibody/pm41407230-147-79-82
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lamp2a - by Bioz Stars, 2026-09
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lysosome-associated membrane protein 2  (CH Instruments)
90
CH Instruments lysosome-associated membrane protein 2
RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and <t>LAMP2</t> proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)
Lysosome Associated Membrane Protein 2, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysosomal+associated+membrane+protein+2/lysosome+associated+membrane+protein+2/pm35090998-274-0-36
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Image Search Results


(A) Schematic representation of three biochemically distinct BAF complexes: nBAF (neuronal cBAF), GBAF, and PBAF. Complex-defining unique subunits are represented in colors. Subunits shared by all three complexes are not indicated. Degraders and small-molecule inhibitors to target each complex are noted below. Schematic was created using BioRender. (B) Neurons were treated with DMSO (control) or the indicated concentrations of ACBI1 or cis -ACBI1 (inactive isomer of ACBI1) for 3 h. Whole-cell lysates were electrophoresed, western blotted, and probed for the indicated BAF subunits. (C–E) Neuronal lysates were used to immunoprecipitate SMARCA4 (C), ARID1A (D), and ARID1B (E). Five percent of the cell lysate was used as input. Histone 3 and IgG are depicted as loading controls. (F and G) Transcriptional assays in which Arc pre-mRNA normalized by GAPDH pre-mRNA levels is illustrated. (F) Neurons were treated with ACBI1 or cis -ACBI1 for 3 h followed by bicuculline and 4AP treatment for 15 min (Bic + 4AP). (G) Neurons were treated with BAF ATPase domain inhibitors BRM014 and FHT for 30 min, followed by Bic + 4AP treatment to induce neuronal activity. Gray dots represent biological replicates, error bars show SE of the mean. * p < 0.05 and ** p < 0.01. One-way ANOVA was followed by Tukey’s post hoc test. Approximate position of the nearest molecular weight marker is depicted against each band.

Journal: Cell reports

Article Title: Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation

doi: 10.1016/j.celrep.2024.114877

Figure Lengend Snippet: (A) Schematic representation of three biochemically distinct BAF complexes: nBAF (neuronal cBAF), GBAF, and PBAF. Complex-defining unique subunits are represented in colors. Subunits shared by all three complexes are not indicated. Degraders and small-molecule inhibitors to target each complex are noted below. Schematic was created using BioRender. (B) Neurons were treated with DMSO (control) or the indicated concentrations of ACBI1 or cis -ACBI1 (inactive isomer of ACBI1) for 3 h. Whole-cell lysates were electrophoresed, western blotted, and probed for the indicated BAF subunits. (C–E) Neuronal lysates were used to immunoprecipitate SMARCA4 (C), ARID1A (D), and ARID1B (E). Five percent of the cell lysate was used as input. Histone 3 and IgG are depicted as loading controls. (F and G) Transcriptional assays in which Arc pre-mRNA normalized by GAPDH pre-mRNA levels is illustrated. (F) Neurons were treated with ACBI1 or cis -ACBI1 for 3 h followed by bicuculline and 4AP treatment for 15 min (Bic + 4AP). (G) Neurons were treated with BAF ATPase domain inhibitors BRM014 and FHT for 30 min, followed by Bic + 4AP treatment to induce neuronal activity. Gray dots represent biological replicates, error bars show SE of the mean. * p < 0.05 and ** p < 0.01. One-way ANOVA was followed by Tukey’s post hoc test. Approximate position of the nearest molecular weight marker is depicted against each band.

Article Snippet: To inhibit BAF ATPase function, BRM014 (compound 14, MedChemExpress; catalog no. HY-119374) and FHT-1015 (MedChemExpress; catalogue no. HY-144896) were used at concentrations described in figure legends.

Techniques: Control, Western Blot, Activity Assay, Molecular Weight, Marker

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation

doi: 10.1016/j.celrep.2024.114877

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: To inhibit BAF ATPase function, BRM014 (compound 14, MedChemExpress; catalog no. HY-119374) and FHT-1015 (MedChemExpress; catalogue no. HY-144896) were used at concentrations described in figure legends.

Techniques: Recombinant, Plasmid Preparation, Magnetic Beads, One Step RT-PCR, SYBR Green Assay, Mass Spectrometry, Software

RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and LAMP2 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)

Journal: Cell Death & Disease

Article Title: RACK1 depletion in the ribosome induces selective translation for non-canonical autophagy

doi: 10.1038/cddis.2017.204

Figure Lengend Snippet: RACK1 depletion-induced autophagy is non-canonical. ( a ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies (left panel). Intensities of Atg5/12 and Beclin1 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted (right panel). ( b ) HT1080 cells were transfected with siRNAs against control or RACK1 in combination with or without Beclin1 siRNA (100 pmol). After 48 h, these cells were analyzed by immunoblotting using the indicated antibodies. ( c ) HT1080 cells were transfected with control or Atg5 siRNA (100 pmol). After 24 h, the cells were re-transfected with control or RACK1 siRNA. After a further 48 h incubation, the cell extracts were subjected to immunoblot analysis using the indicated antibodies. ( d ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 48 h, followed by immunoblot analysis using the indicated antibodies. ( e ) HT1080 cells transfected with control or RACK1 siRNAs (50 pmol) were incubated for 24 h. Extracts of siRNA-transfected cells were pretreated with rapamycin 1 μM for 24 h, followed by immunoblot analysis using the indicated antibodies. Intensities of LAMP1 and LAMP2 proteins were normalized against that of tubulin proteins, and the relative expressions in RACK1 siRNA-treated cells compared with that in control cells were plotted. * P <0.05, ** P <0.01 (Student’s t -test)

Article Snippet: Antibodies against Atg5, Atg9A, Atg4B, Atg16, LAMP1 and LAMP2 were obtained from ProSci (Poway, CA, USA), and the anti-rpS3 antibody was from HAEL (Seoul, Korea).

Techniques: Transfection, Control, Incubation, Western Blot